dna sequencing datasets (Medicago)
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Dna Sequencing Datasets, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+sequencing+datasets/pmc11354556-179-7-6?v=Medicago
Average 90 stars, based on 1 article reviews
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1) Product Images from "Comparative Chloroplast Genomes Analysis Provided Adaptive Evolution Insights in Medicago ruthenica"
Article Title: Comparative Chloroplast Genomes Analysis Provided Adaptive Evolution Insights in Medicago ruthenica
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms25168689
Figure Legend Snippet: Gene contents in 61 complete chloroplast genomes of Medicago ruthenica .
Techniques Used: Functional Assay, Membrane
Figure Legend Snippet: The pan-chloroplast of Medicago ruthenica . Genes shown on the outside of the first outer ring are transcribed counter-clockwise, while those on the inside are transcribed clockwise. Different functional groups of genes are color-coded. The dark grey bars on the second outer ring correspond to GC content.
Techniques Used: Functional Assay
Figure Legend Snippet: The complete chloroplast genome sequences of Medicago ruthenica were visualized with the pan-chloroplast genome as a reference. Gray arrows and thick black lines represent the orientation of genes. Purple bars indicate exons, sky-blue bars denote untranslated regions (UTRs), and red bars highlight non-coding sequences (CNS). Gray bars correspond to mRNA, while white regions show sequence differences among all analyzed chloroplast genomes. The horizontal axis shows the positions within the chloroplast genome, and the vertical scale indicates the identity percentage, ranging from 50% to 100%. ( a ) W1, W2, and W3 are cultivated varieties from the western region, while W4, W6, and W15 are wild varieties from the western region. ( b ) E1, E4, and E5 are cultivated varieties from the eastern region, while E15, E17, and E23 are wild varieties from the eastern region.
Techniques Used: Sequencing, Western Blot
Figure Legend Snippet: The nucleotide diversity (π) values in Medicago ruthenica chloroplast genome. Nucleotide diversity by sliding window analysis in 61 complete chloroplast genomes. Window length: 1000 bp, step size: 200 bp.
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Figure Legend Snippet: The evolutionary values of the Ka/Ks ratio in Medicago ruthenica .
Techniques Used:
Figure Legend Snippet: Phylogenetic relationships and haplotypes of Medicago ruthenica based on complete chloroplast genome sequences. ( a ) The phylogenetic tree was established by the maximum likelihood method, with bootstrap replications set to 1000. Red represents the varieties from the western region, and blue represents the varieties from the eastern region, corresponding to the color scheme throughout the figure. ( b ) Haplotype network of Medicago ruthenica chloroplasts. Each circle in the haplotype network represents a unique haplotype, with the size of the circle proportional to the frequency of the haplotype. Lines connecting the circles indicate mutational steps between haplotypes. The colors within the circles are consistent with those in the phylogenetic tree.
Techniques Used: Western Blot
Figure Legend Snippet: Haplotype classification of 61 Medicago ruthenica chloroplast genomes.
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Figure Legend Snippet: The phylogenetic tree was constructed based on SNPs across the genomes of the Medicago ruthenica . Red represents varieties from the western region, blue represents varieties from the eastern region, and green represents varieties from the intermediate region.
Techniques Used: Construct, Western Blot
Figure Legend Snippet: ( a ) Heatmap of chloroplast gene expression in Medicago ruthenica under abiotic stress. ( b ) Number of upregulated genes under different abiotic stresses. ( c ) Number of downregulated genes under different abiotic stresses.
Techniques Used: Gene Expression
Figure Legend Snippet: RNA editing efficiency in chloroplast genes under abiotic stress in Medicago ruthenica .
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